Curriculum Vitae—George P. Smith

June 2004 

TITLE,  ADDRESS AND PHONE NUMBERS 

Curators Professor Division of Biological Sciences 406 Tucker Hall University of Missouri–Columbia Columbia, MO 65211-7400

Office (573) 882-3344 Lab (573) 882-2720 Fax (573) 882-0123 e-mail smithgp@missouri.edu

DATE AND PLACE OF BIRTH  March 10, 1941, Norwalk, CT 

EDUCATION 

A.B., Biology, Haverford College, 1963

Ph.D., Bacteriology and Immunology, Harvard University, 1970

      Thesis advisor, Dr. Edgar Haber, Mass. General Hospital

Postdoctoral Training, University of Wisconsin, 1970–1975

      Advisor, Dr. Oliver Smithies 


ACADEMIC POSITIONS 


Curators Professor, University of Missouri, September 2000–present

Professor of Biological Sciences, University of Missouri, September 1990–present

Associate Professor of Biological Sciences, University of Missouri, June 1981–Sept 1990

Assistant Professor of Biological Sciences, University of Missouri, July 1975–June 1981 


GRANTS AND FELLOWSHIPS 


N.I.H. Predoctoral Fellowship, 1966–1969

N.I.H. Postdoctoral Fellowship, 1970–1971

Helen Hay Whitney Fellowship, 1971–1974

N.I.H. Grant RO1 GM 22686 “Molecular Genetics of Antibodies” 9/75–8/78

N.I.H. Grant RO1 GM 25626 “Molecular Genetics of Nematode Development” 9/78–8/81

N.I.H. Grant RO1 AI 20565 “Filamentous Phage Physiology” 9/84–8/87

Supplement to above grant 9/85–8/87

N.I.H. Grant RO1 GM41478 “Filamentous Fusion Phage” 7/89–3/94

Army Research Office Grant DAAL03-92-G-0178 “Epitope Discovery: A New Approach to Vaccines” 7/92–6/97.

Supplement to above for Landscape Libraries 1995

N.I.H. STTR Phase I “Diagnostic Peptides for Lyme Disease”

Kosan Biosciences special grant “Diagnostic Peptides for Lyme Disease and other Diseases” 7/97-6/98

N.I.H. Grant RO2 GM41478 “Epitope Discovery: A New Route to Vaccines”  7/97-3/05

CDC. Contract – “Epitope Discovery to Identify Peptides Diagnostic for Chronic Fatigue Syndrome” – May 2001–April 2003

NIH Center Grant P50 CA 103130—“Center for Single Photon-Emitting Cancer Imaging Agents” (Wynn Volkert, PI; G.P. Smith PI for Biotechnology Core)—8/1/03–7/31/08

UMC Research Council Grant BR2728 “Filamentous Phage Physiology” 1983–4

UMC Research Council Grant BR3288 “Filamentous Phage Physiology” 9/87–8/88

UMC Research Leave Funded for 1983–4 academic year at Duke University with Robert Webster

UMC Weldon Spring Grant “A Paratope Library” Awarded 1989, but declined because theN.I.H. Grant  GM41478 was funded.

University of Missouri Research Board Grant “A Landscape Library” Awarded 1995 but declined because of supplement to Army Research Office grant noted above

University of Missouri Research Board Grant “Platelet-activating phage: a new route to hemostatics”  Awarded September 1999 


PUBLICATIONS 


Smith, G.P., Hood, L., and Fitch, W.M.  1971.  Antibody diversity.  Ann. Rev. Biochem.  40, 969–1021.

Smith, G.P. 1973. The Variation and Adaptive Expression of Antibodies.  Harvard University Press, Cambridge, Mass.

Smith, G.P.  1973.  Mouse immunoglobulin kappa chain MPC 11: extra amino-terminal residues. Science 181, 941–943.

Smith, G.P. 1973. Unequal crossover and the evolution of multigene families. Cold Spring Harbor Symp. Quant. Biol. 38, 507–513.

Bishop, J.O., and Smith, G.P.  1974.  The assessment of RNA homogeneity by molecular hybridization. Cell 3, 341–346.

McKean, D.J., and Smith, G.P. 1974.  The use of radiolabelled T₁ ribonuclease to monitor the efficiency of Edman degradations.  Biochem. J. 139, 779–780.

Smith, G.P. 1976. Unequal crossover and the evolution of repeated DNA sequences. Science 191, 528–535.

Smith, G.P. 1977. The significance of hybridization kinetic experiments for theories of antibody diversity.  Cold Spring Harbor Symp. Quant. Biol.  41, 863–875.

Rose, S.M., Kuehl, W.M., and Smith, G.P.  1977.  Cloned MPC 11 myeloma cells express two kappa genes: a gene for a complete light chain and a gene for a constant-region fragment.  Cell 12, 453–462.

Smith, G.P. 1978. Sequence of the full-length immunoglobulin kappa-chain of mouse myeloma MPC 11.  Biochem. J.  171, 337–347.

Smith, G.P.  1978.  What is the origin and evolution of repetitive DNAs?  Trends in Biochem. Sci. 3, 34–36.

Smith, G.P.  1978.  Non-Darwinian evolution and the beard of life.  Stadler Symp.  10, 105–110.

Zacher, A.N., Stock, C.A., Golden, J.W., and Smith, G.P.  1980.  A new filamentous phage cloning vector: fd-tet.  Gene  9, 127–140.

Nelson, F.K., Friedman, S.M., and Smith, G.P. 1981. Filamentous phage DNA cloning vectors: a noninfective mutant with a nonpolar deletion in gene III.  Virology 108, 338–350.

Crissman, J.W. and G.P. Smith. 1984. Gene-III protein of filamentous phages: evidence for a carboxyl-terminal domain with a role in morphogenesis. Virology 132, 445–455.

Smith, G.P. 1985.  Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228, 1315–1317.

Scott, J.K., G.P. Smith, L.S. Lerman and J. Gerlach.  1985.  Cataloging germline immunoglobulin V_l genes by direct analysis of cellular DNA.  In Advanced in Gene Technology, Vol II: The Molecular Biology of the Immune System (Streilein, J.W., Ahmad, F., Black, S., Blomberg, B.,and Woellmy, R.W., eds.)  Cambridge University Press, pp. 289–290.  (ABSTRACT)

Smith, G.P.  1987.  Filamentous phages as cloning vectors.  In Vectors: A Survey of Molecular Cloning Vectors and their Uses (R. Rodriguez and D. Denhardt, eds.)  Stoneham, MA, Butterworth, pp. 61–85.

Michiels, F., Craig, A.G., Zehetner, G., Smith, G.P. and Lehrach, H. 1987. Molecular approaches to genome analysis: a strategy for the construction of ordered overlapping clone libraries.  Computer Applications in the Biosciences 3, 203–210.

Smith, G.P. 1988.  Filamentous phage assembly: morphogenetically defective mutants that do not kill the host.  Virology 167, 155–165.

Bauer, M., and G.P. Smith  1988.  Filamentous phage morphogenetic signal sequence and orientation of DNA in the virion and gene-V protein complex.  Virology 167, 166–175.

Parmley, S.P., and G. P. Smith  1988.  Antibody-selectable filamentous fd phage vectors: affinity purification of target genes.  Gene 73, 305–318.

Scott, J.K., and G.P. Smith  1990.  Searching for peptide ligands with an epitope library.  Science 249, 386–390.

Smith, G.P. 1991.  Surface presentation of protein epitopes using bacteriophage expression systems. Currrent Opinions in Biotechnology 2, 668–673.

Smith, G.P. 1993. Antibody screening of DNA expression libraries. In Encyclopedia of Immunology (I.M. Roitt and P.J. Delves, Eds.) Saunders, London.  pp. 102–104.

Smith, G.P., and J.K. Scott 1993.  Libraries of peptides and proteins displayed on filamentousphage.  In Methods Enzymol. 217, 228–257.

Smith, G.P., D.A. Schultz and J.E. Ladbury  1993.  A ribonuclease S-peptide antagonist discovered with a bacteriophage display library.  Gene 128, 37–42.

Smith, G.P.  1993.  Preface: Surface display and peptide libraries.  Gene 128, 1–2.  (PREFACE TO AN ISSUE OF THE JOURNAL DEVOTED TO PHAGE-DISPLAY AND RELATED TECHNOLOGY)

Haas, S.J., and G.P. Smith 1993.  Rapid sequencing of viral DNA from filamentous bacteriophage. Biotechniques 15, 422–431.

Zhong, G., and G.P. Smith 1994.  Kinetic microplate assay for titering microbial cells. Biotechniques 16, 838–839.

Zhong, G., Smith, G.P., Berry, J., and R.C. Brunham 1994.  Conformational mimicry of a chlamydial neutralization epitope on filamentous phage. J. Biol. Chem. 269, 24183–24188.

Smith, G.P. 1994.  The progeny of sexual PCR.  Nature 370, 321–322.

Yu, J. and Smith, G.P. 1996. Affinity maturation of phage-displayed peptide ligands.  Methods Enzymol. 267, 3–27

Petrenko, V.A., Smith, G.P., Gong, X., and Quinn, T.  1996.  A library of organic landscapes on filamentous phage.  Protein Engineering  9, 797–801.

Smith, G.P., and Yu, J. 1996.  In search of dark horses: affinity maturation of phage-displayed ligands.  Molecular Diversity 2, 2–4.

Smith, G.P., and Petrenko, V.A. 1997.  Phage display.  Chem. Rev. 97, 391–410.

Smith, G.P., Petrenko, V.A., and Matthews, L.J. 1998.  Cross-linked filamentous phage as an affinity matrix.  J. Imm. Meth. 215, 151–161.

Li, J., Smith, G.P., and Walker, J.C. 1999.  Kinase interaction domain of kinase-associated protein phosphatase, a phosphoprotein-binding domain.  Proc. Natl. Acad. Sci. USA 96, 7821–7826.

Petrenko, V.A., and Smith, G.P.  2000.  Phages from landscape libraries as substitute antibodies.  Protein Engineering 13, 589–592.

Kouzmitcheva, G., Petrenko, V.A., and Smith, G.P.  2001.  Diagnostic peptides for Lyme disease through epitope discovery.  Clin. Diagn. Lab. Immunol. 8, 150–160.

Matthews, L.J., Davis, R., and Smith, G.P.  2002.  Immunogenically fit subunit vaccine components via epitope discovery from natural peptide libraries.  J. Immunol. 169, 837–846.

Smith, G.P. and Fernández, A.M.  2004.  Effect of DNA copy number on genetic stability of phage-displayed peptides.  BioTechniques 36, 610–619.

 

INVITED SEMINARS

 


Cold Spring Harbor Symposium, 1976 (invited speaker; see Smith, 1977)

Washington University, Microbiology Department, 1977

University of Utah, Microbiology Department, 1978

Gordon Conference in Theoretical Biology, 1978 (organizer and moderator of session on evolution of repetitive DNA)

University of North Carolina, Microbiology, 1979

Rockefeller University, 1980

Aquisition, Integration, and Analysis of Molecular Genetic Information Using Modern Computational  Techniques.  International Workshop, Aspen Center for Physics, August–Sept. 1982  (Invited Presentation: “Ordered Clone Libraries”)

Duke University Medical School, Biochemistry Department, 1984

Bacteriophage Meeting, Cold Spring Harbor, 1984 (Presentation: “Filamentous Fusion Phage”)

Macromolecules, Genes and Computers: International Symposium and Workshop.  Waterville Valley, NH 1986 (Invited Presentation: “Deep Principles in the Reconstruction of Evolutonary Trees from Nucleotide Sequence Data”)

Syntro Corp., San Diego, 1986

Prokaryotic Gene Regulation, Cold Spring Harbor, 1987 [Presentation: “The Morphogentic Signal Sequence mos and the Orientation of DNA in Filamentous Phage” (with Margaret Bauer); Poster: “Filamentous Phage as an Antibody-Selectable Expression  Vector” (with Steve Parmley)]

Genome Mapping and Sequencing Meeting, Cold Spring Harbor, 1988 (Poster: “The Lehrach Probe Hybridization Map: Algebra of Consistency and Statistics of Inconsistencies”)

Advanced Bacterial Genetics Course, Cold Spring Harbor, 1987: Guest Lecturer

Cetus Corporation, December 1988, June 1990

Genelabs Incorporated, December 1988

Genex Corporation, January 1989

Affymax Research Institute, June 1989

Synthetic Peptides: Approaches to Biological Problems.  UCLA Symposium, Feb–March 1990 (Invited presentation: “An Epitope Library”)

Banbury Conference on Vectors for Cloning the Immune Response. Cold Spring Harbor, April, 1990: Invited participant

Protos Corporation, June 1990

Kansas State University, September 1990

Monsanto Corporation, September 1990

Washington University (St. Louis) November 1990

University of Kansas Medical School, November 1990

Abbott Laboratories, December 1990

CIBA-Geigy Corporation, March 1991

North Carolina State University, March 1991

Keystone Symposium on Protein Folding, April 1991  (Invited presentation: Cloning small protein domains in filamentous phage)

Twelfth American Peptide Symposium, June 1991 (Invited presentation: Using an epitope library to identify peptide ligands for antibodies against folded peptides)

New England Biolabs, December 1991

Marquette Life Sciences Symposium, March 1992 (Invited presentation: Epitope libraries and their applications)

Banbury Conference on Phage Display, Cold Spring Harbor, April 1992 (Co-organizer)

Science Innovation 92, San Francisco, July 1992 (Invited Plenary Speaker)

Gordon Conference on Biomolecular Recognition and Immobilization, Brewster Academy, August 1992 (Invited participant)

University of Missouri–Kansas City, October 1992

Symposium on Pharmaceutical Design Using Epitope Selection Technologies, Palo Alto Institute for Molecular Medicine, February 1993 (Invited speaker)

United Biomedical, Inc., February 1993

Keystone Symposium on Progress in Drug Design Based on Peptides and Proteins, March 1993 (Invited speaker)

Michigan State University, February 1994

University of Texas Houston Medical Center, February 1994

Washington State University, March 1994

Simon Fraser University, Vancouver, BC, March 1994

Symposium and Workshop on Searching Sequence Space, Santa Fe Institute, Santa Fe, NM, May 1994 (invited speaker and participant)

Symposium in Biological Sciences, University of Rochester, May 1994 (invited speaker)

Symposium on Molecular Diversity, North Carolina Biotechnology Center, Research Triangle Park, NC, June 1994 (invited speaker)

Course on Phage Display Technology, Paris, France, July 22–31, 1994 (instructor and invited speaker)

University of Nebraska Medical School, September 1994

International Symposium on  Combinatorial Libraries, Maratea, Italy, May 1995 (invited speaker)

Phage Display Symposium and Workshop, NIH, October 27, 1995

International Conference on Combinatorial Library Methods for Basic Research and Drug Discovery, Tucson, AZ December 2–4, 1995 (program committee and invited speaker)

Educational session on Combinatorial Library Methods for Basic Research and Drug Discovery, Annual meeting of the American Association for Cancer Research, Washington, DC, April 20, 1996; invited speaker

International symposium on Antibody Technology and Applications in Health and Environment, Brauschweig, Germany, September 6–10, 1996; invited speaker

Department of Pharmacology and Toxicology, Dartmouth Medical School, January 15, 1997

Department of Medical Microbiology, University of Manitoba, February 12–13, 1997

Kosan Biosciences, Inc., Burlingame, CA, March 13, 1997

European Phage Club, Smolenice, Slovak Republic, May 24–26, 1997; invited speaker

Second International Conference on Combinatorial Library Methods for Basic Research and Drug Discovery, Tucson, AZ  January 11–12, 1999.

Sigma Chemical Co. June 11, 1999

Instituto di Ricerche di Biologia Moleculare, Rome, Italy  March 27, 2000

Banbury Conference on Strategies for Identification and Characterization of Unknown Pathogens, April 2–5, 2000

Eli Lilly, Indianapolis, IN May 22, 2000

UTOPIA 2000: Understanding The Output Of Protein-Protein Interaction Analyis, International Science Forum; Heidelberg, Germany  July 4–5, 2000

International conference Understanding Phage Display – Structure, Biology, and Applications, Vancouver, BC, September 21–23, 2000; invited speaker

Nobel Jubilee Symposium in Chemistry Frontiers in Molecular Sciences, Stockholm, Sweden, December 4–6, 2001; invited speaker.  Title: Selection versus design in chemical engineering

International conference Understanding Phage Display, Vancouver, BC, January 17–20, 2003; co-organizer

International Antibody Engineering Conference, San Diego, CA, November 30–December 3, 2003; invited speaker 
 


 


TEACHING (at University of Missouri–Columbia) 





Year	Sem	Course	Title	Enrollment
1975	W	1	General Biology	50(?)
1976	F	202	General Genetics	91
1977	W	452	Nucleic Acids	12
	F	1	General Biology	22
	F	238	Basic Genetics I	20
1978	W	239	Basic Genetics II	17
	F	374	Cell Biology I	12
	F	238	Basic Genetics I	17
1979	W	239	Basic Genetics II
	F	238	Basic Genetics I
	F	1	General Biology	170
1980	W	239	Basic Genetics II	17
	F	238	Basic Genetics I	15
	F	374	Cell Biology I Laboratory	12
1981	W	239	Basic Genetics II	9
	W	411	Cell Biology Seminar	12
	F	238	Basic Genetics I	23
1981	F	Developed two-week laboratory exercise in genetic engineering for Biology 1 Honors class.  This exercise was used for the first time Oct., 1981
1982	W	407	Molecular Genetics Lab	12
	W	239	Basic Genetics II	20
	F	1GH	General Biology Honors	24
1983	W	239	Basic Genetics II	15
1983–1984, Research Leave
1985	W	407	Molecular Genetics Lab	14
	W	1GH	General Biology Honors	16
	F	407	Molecular Genetics Lab	14
1986	W	203	Intro. to Cell Biology	75
	F	407	Molecular Genetics Lab	12
1987	W	202	General Genetics (Writing Intensive)	80
	F	407	Molecular Genetics Lab	16
1988	F	407	Molecular Genetics Lab	16
1989	W	202	General Genetics	77
	F	300	Molecular Biology Lab	20
1990	W	301	Molecular Biology Lab	16
	F	374	Molecular Biology Lab
1991	W	374	Molecular Biology Lab	7
	F	374	Molecular Biology Lab	8
1992	F	374	Molecular Biology Lab	13

 




Year	Sem	Course	Title	Enrollment
1993	F	374	Molecular Biology Lab	4
	F	241	Genetics Lab (teamed with Golomb)	20
1994	F	370	Molecular Genetics Lecture	41
1995	W	370	Molecular Genetics Lecture	23
1995	F	370	Molecular Genetics Lecture	45
1996	W	202	General Genetics	95
1996	F	370	Molecular Genetics Lecture	38
1997	F	202	General Genetics	270
1999	W	370	Molecular Biology Lecture	45
1999	F	202	General Genetics (Honors)	33
2000	W	370	Molecular Biology Lecture	47
2001	W	370	Molecular Biology Lecture	38
2001	F	370	Molecular Biology Lecture	28
2002	W	370	Molecular Biology Lecture	40
2002	F	370	Molecular Biology Lecture	40
2003	W	370	Molecular Biology Lecture	27
2004	W	370	Molecular Biology Lecture	39

 


POSTDOCTORAL ADVISEES 


Scott, J.K.   July 1989–June 1992.  Received NIH NRSA Postdoctoral Fellowship for July 1990–June 1992.  Currently Associate Professer, Simon Fraser University, Barnaby, British Columbia, Canada

Guangming Zhong   December 1992–December 1993; currently Associate Professor, University of Manitoba, Winnipeg, Manitoba, Canada

Ed Lancy   January 1993–March 1994; currently an optometrist

Galina Kouznicheva  April 1, 1997–May 1999; currently at Auburn University

Leslie J. Matthews  August 1998–July 2003

Melissa Nevils  January 2002–present 


VISITING PROFESSOR 


Valery Petrenko, Research Professor, Novosibirsk, Russia  October 1993–June 1996; Research Professor, University of Missouri, 1996–2000 (took position of Professor at Auburn University, October, 2000) 


GRADUATE STUDENT ADVISEES 


Zacher, A.N.  M.A.   Fall 1977–Spring 1979  (finished master's research project (Zacher et al., 1980) but left to go to medical school before writing thesis)

Stock, C.A.  M.A.   Jan. 1978–Aug. 1981  M.A. awarded August 1981

Scott, J.K.   Ph.D.   Jan. 1980–May, 1985   Ph.D. awarded May 1985

Crissman, J.W.   M.A.   Aug. 1981–Aug.1983   M.A. awarded August 1981

Parmley, S.F.   Ph.D.   Aug. 1984–Dec. 1988   Ph.D. awarded Dec. 1988

Bauer, M.   M.A.   Jan. 1986–August 1988   M.A. awarded August 1988

Zubairu, Kabir   M.A.   April 1987–June 1989  (left to go to medical school at University of Michigan)

Fernandez, A.M.   M.A.   Fall 1991–1995  M.A. awarded 1995

Choukri, S.   Ph.D.  August 1992–December 1999  Ph.D. awarded December 1999

Yu, J.   Ph.D.   August 1992–August 1995   Ph.D. awarded August 1995

Matthews, L.J.   Ph.D.   August 1995–August 1998  Ph.D. awarded August 1998 


OTHER DOCTORAL STUDENT COMMITTEES (1997–2003) 


Nancy Vosnidou (Biological Sciences)

Yeongseo Yeo (Philosophy)

Jun Zou (Biochemistry)

Jody Berry (University of Manitoba, Winnipeg)

Melissa Nevils (Veterinary Pathobiology)

Dave Taylor (Biochemistry)

Spencer Leigh (Molecular Microbiology and Immunology)

Anjali Dogra (Biological Sciences) 


OTHER UNIVERSITY AND PROFESSIONAL SERVICE 


DBS Curriculum and Budget Committees.

Research Council 1986–1989

Search Committee for Cell Biology (Chair)

Search Committee for Developmental Molecular Biology

Search Committee for Microbiology

Reviewed about 160 papers for various journals.

Reviewed one grant proposal for NSF

NIH ad hoc study sections (about 1–2 per year).

Organized DNA Sequencing Workshop, with guest demonstrator Nigel Godson:  12 participants,1979.

Organized In situ Hybridization Workshop, with guest demonstrators David Ward and Pennina Langer, May 1981.

Organized University Assembly Speakers lecture on Bayesian Philosophy and Workshop on Bayesian Statistics, with lecturer and demonstrator Professor D.V. Lindley; March 10–12, 1982.

Co-organized and taught Western Blot Workshop with Dr. Kim Wise, 12 participants, 1983.

Organized Arts and Science Week Distinguished Lectureship by Professor Edwin T. Jaynes, February 21–23, 1989

Molecular Biology Curriculum Committee, 1989

Search Committee, Director of Protein Sequencing Lab, 1990

Editor, Protein Engineering (1993–present)

Search committee for immunologist, Department of Medical Microbiology and Immunology 1995–1996